Surface-displayed expression of a neutralizing epitope of Apx2A exotoxin in Saccharomyces cerevisiae and oral administration of it for protective immune responses against challenge by Actinobacillus pleuropneumoniae

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  • KIM Jung-Mi
    Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
  • JUNG Dea-Im
    Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
  • EOM Yoo Jeong
    Korea Minjok Leadership Academy
  • PARK Seung-Moon
    Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
  • YOO Han-Sang
    College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute and BK21 for Veterinary Science, Seoul National University
  • JANG Yong-Suk
    Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
  • YANG Moon-Sik
    Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University
  • KIM Dae-Hyuk
    Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University

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タイトル別名
  • Surface-Displayed Expression of a Neutralizing Epitope of ApxIIA Exotoxin in Saccharomyces cerevisiae and Oral Administration of It for Protective Immune Responses against Challenge by Actinobacillus pleuropneumoniae
  • Surface-Displayed Expression of a Neutralizing Epitope of ApxIIA Exotoxin in<i>Saccharomyces cerevisiae</i>and Oral Administration of It for Protective Immune Responses against Challenge by<i>Actinobacillus pleuropneumoniae</i>

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A neutralizing epitope fragment of ApxIIA toxin (ApxIIA#5) of the Korean Actinobacillus pleuropneumoniae serotype 2 strain was expressed and immobilized on the cell surface of Saccharomyces cerevisiae for efficient vaccine development. Expression of ApxIIA#5 was confirmed by Western blot analysis using cell-wall proteins, and the surface display of ApxIIA#5 was further visualized under confocal microscopy. Quantitative ELISA revealed that the recombinant ApxIIA#5 directed to the cell surface consisted of approximately 16% cell-wall proteins, estimated to be 35 mg of ApxIIA#5 protein per liter of cultured cells. An immunoassay revealed that antigen-specific antibodies against ApxIIA#5 were present in the sera of mice fed recombinant ApxIIA#5-expressing yeast, but not in mice fed the wild-type nor the vector-only transformant. Moreover, the mice fed the recombinant epitope-expressing yeast were protected from injection of a lethal dose of A. pleuropneumoniae.

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