Digestion and Gastrointestinal Absorption of the 14-16-kDa Rice Allergens

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  • YAMADA Chikako
    School of Nutritional Sciences, Nagoya University of Arts and Sciences
  • YAMASHITA Yukari
    School of Nutritional Sciences, Nagoya University of Arts and Sciences
  • SEKI Ryotaro
    School of Nutritional Sciences, Nagoya University of Arts and Sciences
  • IZUMI Hidehiko
    School of Nutritional Sciences, Nagoya University of Arts and Sciences
  • MATSUDA Tsukasa
    Graduate School of Bioagricultural Sciences, Nagoya University
  • KATO Yasuko
    Department of Clinical Nutrition, Kawasaki University of Medical Welfare

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The digestibility and gastrointestinal absorption of 14–16-kDa rice allergens (RAs) were investigated. RAs and bovine serum albumin (BSA) were first evaluated for their digestibility. BSA was digested completely by in vitro incubation with some proteases, but RAs remained almost intact. Administered orally (20 mg per mouse), intact RAs were clearly detected in the small intestine even 60 min after the administration, the amount of total RAs in the small intestine being estimated to be 0.59 mg. RAs were then biotinylated and infused into the duodenal lumen of anesthetized mice, and portal blood was collected. The RA concentrations in the portal plasma were respectively estimated to be 0.4–0.9 and 0.3–2.5 μg/ml for 0.4 and 4 mg doses. These results suggest that RAs are highly resistant to digestive enzymes and that about 1/100 of orally administered RAs remain intact in the small intestine, while at least 1/1,000–1/10,000 is absorbed and delivered into circulated blood.

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