Contribution Ratios of <i>amyA</i>, <i>amyB</i>, <i>amyC</i> Genes to High-Level α-Amylase Expression in <i>Aspergillus oryzae</i>
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- NEMOTO Takashi
- Department of Biotechnology, The University of Tokyo
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- MARUYAMA Jun-ichi
- Department of Biotechnology, The University of Tokyo
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- KITAMOTO Katsuhiko
- Department of Biotechnology, The University of Tokyo
Bibliographic Information
- Other Title
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- Contribution Ratios of amyA, amyB, amyC Genes to High-Level α-Amylase Expression in Aspergillus oryzae
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Abstract
Aspergillus oryzae strains express α-amylases abundantly, and the genome reference strain RIB40 has three α-amylase genes (amyA, amyB, and amyC). However, there is no information on the contribution ratios of individual α-amylase genes to total expression. In this study, we generated single, double, and triple disruptants of α-amylase genes by employing a strain (ΔligD) with high gene-targeting efficiency and pyrG marker recycling in A. oryzae. All the disruptants showed reduced activities of α-amylases, and the triple disruptant completely lost activity. Comparative analyses of the activities and mRNA amounts of the α-amylases suggest that the contribution of amyA to the α-amylase expression is smaller than those of amyB and amyC. The present study suggests that the ability to express a large amount of α-amylases in A. oryzae is attributed to gene duplication of genes such as amyB and amyC.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (8), 1477-1483, 2012
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206479542912
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- NII Article ID
- 130004137918
- 10031065836
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC38fmslOmtw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 023915602
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- PubMed
- 22878189
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed