Stereoselective Incorporation of Isoleucine into Cypridina Luciferin in Cypridina hilgendorfii (Vargula hilgendorfii)
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- KATO Shin-ichi
- Graduate School of Bioagricultural Sciences, Nagoya University
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- OBA Yuichi
- Graduate School of Bioagricultural Sciences, Nagoya University
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- OJIKA Makoto
- Graduate School of Bioagricultural Sciences, Nagoya University
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- INOUYE Satoshi
- Yokohama Research Center, Chisso Co.
Bibliographic Information
- Other Title
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- Stereoselective Incorporation of Isoleucine into Cypridina Luciferin in<i>Cypridina hilgendorfii</i>(<i>Vargula hilgendorfii</i>)
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Abstract
The emission of light in the marine ostracod Cypridina hilgendorfii (presently Vargula hilgendorfii) is produced by the Cypridina luciferin-luciferase reaction in the presence of molecular oxygen. Cypridina luciferin has an asymmetric carbon derived from isoleucine, and the absolute configuration is identical to the C-3 position in L-isoleucine or D-alloisoleucine. To determine the stereoselective incorporation of the isoleucine isomers (L-isoleucine, D-isoleucine, L-alloisoleucine, and D-alloisoleucine), we synthesized four 2H-labeled isoleucine isomers and examined their incorporation into Cypridina luciferin by feeding experiments. Judging by these results, L-isoleucine is predominantly incorporated into Cypridina luciferin. This suggests that the isoleucine unit of Cypridina luciferin is derived from L-isoleucine, but not from D-alloisoleucine.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (6), 1528-1532, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206479604992
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- NII Article ID
- 10018530585
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7959146
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed