Structure and characteristics of an endo-β-1,4-glucanase, isolated from Trametes hirsuta, with high degradation to crystalline cellulose
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- NOZAKI Kouichi
- Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
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- SEKI Takahiro
- Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
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- MATSUI Keiko
- Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
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- MIZUNO Masahiro
- Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
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- KANDA Takahisa
- Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
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- AMANO Yoshihiko
- Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
書誌事項
- タイトル別名
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- Structure and Characteristics of an Endo-.BETA.-1,4-glucanase, Isolated from Trametes hirsuta, with High Degradation to Crystalline Cellulose
- Structure and characteristics of an endo v 1 4 glucanase isolated from Trametes hirsuta with high degradation to crystalline cellulose
- Structure and Characteristics of an Endo-β-1,4-glucanase, Isolated from<i>Trametes hirsuta</i>, with High Degradation to Crystalline Cellulose
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抄録
Trametes hirsuta produced cellulose-degrading enzymes when it was grown in a cellulosic medium such as Avicel or wheat bran. An endo-β-1,4-glucanase (ThEG) was purified from the culture filtrate, and the gene and the cDNA were isolated. The gene consisted of an open reading frame encoding 384 amino acids, interrupted by 11 introns. The whole sequence showed high homology with that of family 5 glycoside hydrolase. The properties of the recombinant enzyme (rEG) in Aspergillus oryzae were compared with those of the En-1 from Irpex lacteus, which showed the highest homology among all the endoglucanases reported. The rEG activity against Avicel was about 8 times higher than that of En-1 when based on CMC degradation. A remarkable structural difference between the two enzymes was the length of the linker connecting the cellulose-binding domain to the catalytic domain.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (10), 2375-2382, 2007
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479618816
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- NII論文ID
- 10027519502
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8980374
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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