Differentiation of Mouse P19 Embryonic Carcinoma Stem Cells Injected into an Empty Zebrafish Egg Chorion in a Microfluidic Device
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- LEE Jin-Woo
- Department of Electrical Engineering, Korea University
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- NA Dae-Seok
- Department of Electrical Engineering, Korea University
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- KANG Ji-Yoon
- BioMEMS Laboratory, Microsystem Research Center, Korea Institute of Science Technology
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- LEE Sang-Ho
- School of Life Sciences and Biotechnology, Korea University
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- JU Byeong-Kwon
- Department of Electrical Engineering, Korea University
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Mouse P19 embryonic carcinoma (EC) stem cells were xenotransplanted into the emptied chorion, the transparent envelope of a fertilized zebrafish egg (rather than mouse native zona pellucida) combined with a microfluidic device to study P19 EC cell differentiation in the chorion biomaterial. A distilled-water jet was used to remove the innate yolk and perivitelline inner mass from the chorion. P19 EC cells were injected into the emptied chorion using a micropipette, and they were subsequently cultured until the inner space of the chorion became completely occupied by cells. A simple microfluidic device was used for handling convenience and effective experiment. At d15, we found neural cells in the outer layer of the cell mass and beating cardiomyocytes in the inner layer of the large embryoid body. We propose that even though the species are different, the external innate membranes developed for embryo protection represent a useful type of ECM.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (6), 1325-1330, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479709184
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- NII論文ID
- 10018529836
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7958781
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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