UVC Mutagenicity Is Suppressed in Japanese Miso-Treated Human RSa Cells, Possibly via GRP78 Expression

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  • JIANG Xia
    Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University
  • REN Qian
    Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University
  • CHEN Shi-Ping
    Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University
  • TONG Xiao-Bo
    Department of Physiology, Faculty of Basic Medical Sciences, Chengde Medical University
  • DONG Mei
    Department of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine
  • SUGAYA Shigeru
    Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University
  • TANAKA Takeshi
    Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University
  • KITA Kazuko
    Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University
  • SUZUKI Nobuo
    Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

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  • UVC Mutagenicity Is Suppressed in Japanese Miso-Treated Human RSa Cells, Possibly<i>via</i>GRP78 Expression

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Abstract

Little is known about the ability of miso, to modulate mutability in human cells. We have observed increased levels of glucose-regulated protein 78 (GRP78) expression in association with suppression of mutation in human RSa cells irradiated with ultraviolet C (UVC). Here we examined to determine whether miso treatment results in increased GRP78 expression and suppression of UVC mutagenicity in RSa cells. Supernatants of water extracts of miso products and their components were tested. In the sample-treated cells, the amount of GRP78, as estimated by RT-PCR and immunoblotting analysis, increased, and the UVC-induced ouabain resistant mutation (OuaR) and the K-ras codon 12-base substitution mutation frequency decreased. This decrease was not observed in cells with downregulation of GRP78 by GRP78 siRNA transfection. The results suggest that miso suppresses UVC mutagenicity by increasing GRP78 expression in human cells.

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