Temperature-Dependent Self-Splicing Group I Introns in the Flagellin Genes of the Thermophilic<i>Bacillus</i>Species

  • HAYAKAWA Jumpei
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
  • ISHIZUKA Morio
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University

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  • Temperature-Dependent Self-Splicing Group I Introns in the Flagellin Genes of the Thermophilic Bacillus Species

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A previous report described the presence of a self-splicing group I intron in a flagellin gene from a thermophilic Bacillus species. Here, we present evidence that the splicing reaction of the flagellin introns is dependent on temperature. Furthermore, a complementation analysis using a Bacillus subtilis flagellin-deficient mutant indicated that the intron-containing flagellin gene significantly restored the motility of the mutant at higher temperatures.

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