Establishment of a New Detection System for the Dimerization of IRE1α by BiFC Assay

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  • SHINJO Satoko
    Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
  • TASHIRO Etsu
    Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
  • IMOTO Masaya
    Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University

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We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

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