Functional Differentiation of Chitin Synthases in Yarrowia lipolytica
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- SHENG Wei
- Department of Biotechnology, The University of Tokyo
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- YAMASHITA Shuichi
- Department of Agricultural and Environmental Biology, The University of Tokyo
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- OHTA Akinori
- Department of Biotechnology, The University of Tokyo
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- HORIUCHI Hiroyuki
- Department of Biotechnology, The University of Tokyo
書誌事項
- タイトル別名
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- Functional Differentiation of Chitin Synthases in <i>Yarrowia lipolytica</i>
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In this study, we identified seven chitin synthase-encoding genes in the genome of the dimorphic yeast Yarrowia lipolytica. Three encoded chitin synthases with myosin motor-like domains at their N-termini, and we designated these CSM1 to CSM3, whereas four were identified as CHS1 to CHS4. To investigate the functions of these seven genes, we constructed and characterized their deletion mutants. The chs2Δ mutant formed chained cells in which daughter cells were connected with mother cells and had abnormally thick septa at the bud neck. The chs4Δ mutant showed remarkably reduced chitin content in its cell wall. The chs2Δ, csm1Δ, and csm2Δ mutants were found to be highly sensitive to chitin binding dyes, calcofluor white (CFW) and Congo red, whereas the chs4Δ mutant was resistant to CFW. These results suggest that Chs2 and Chs4 play major roles in septum formation and cell wall chitin synthesis respectively, whereas Csm1 and Csm2 are involved in the maintenance of cell wall architecture and/or cell wall integrity. The populations of filamentous cells, a type of cell population that are defined by the lengths of the cellular long and short axes, decreased in the chs3Δ mutant, suggesting that Chs3 is involved in cellular morphogenesis.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (6), 1275-1281, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479803520
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- NII論文ID
- 10031184694
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sjhtFWgsw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024647131
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- PubMed
- 23748777
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可