Mandelate Racemase and Mandelate Dehydrogenase Coexpressed Recombinant Escherichia coli in the Synthesis of Benzoylformate

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  • LI Dali
    Department of Bioengineering, Nanjing University of Science and Technology
  • ZENG Zhen
    Department of Bioengineering, Nanjing University of Science and Technology
  • YANG Junfang
    Department of Bioengineering, Nanjing University of Science and Technology
  • WANG Peng
    Department of Bioengineering, Nanjing University of Science and Technology
  • JIANG Lei
    Department of Bioengineering, Nanjing University of Science and Technology
  • FENG Jing
    Department of Bioengineering, Nanjing University of Science and Technology
  • YANG Chengli
    Department of Bioengineering, Nanjing University of Science and Technology Department of Microbiology, Key Laboratory for Microbiological Engineering of the Agricultural Environment of the Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University

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  • Mandelate Racemase and Mandelate Dehydrogenase Coexpressed Recombinant <i>Escherichia coli</i> in the Synthesis of Benzoylformate

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Benzoylformate is used widely as an organic synthetic intermediate. Mandelate racemase and mandelate dehydrogenase are two enzymes used in producing benzoylformate from racemic mandelate in microbial metabolism. In the present work, the genes encoding mandelate racemase and mandelate dehydrogenase from Pseudomanas aeruginosa strain NUST were cloned and expressed in Escherichia coli to convert racemic mandelate to benzoylformate. The use of whole resting cells of recombinant E. coli allowed the conversion of 65.7 mM solution of racemic mandelate to benzoylformate at a yield of 98.9% in 45 h. The process is a promising alternative for the production of benzoylformate.

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