Functional Analysis of an Endo-1,6-.BETA.-D-glucanase Gene (neg-1) from Neurospora crassa

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  • OYAMA Seiji
    Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
  • INOUE Hirokazu
    Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University
  • YAMAGATA Youhei
    Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
  • NAKAJIMA Tasuku
    Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
  • ABE Keietu
    Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

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  • Functional Analysis of an Endo-1,6-β-D-glucanase Gene(neg-1) from Neurospora crassa
  • Functional Analysis of an Endo 1 6 ベータ D glucanase Gene neg 1 from Neurospora crassa
  • Functional Analysis of an Endo-1,6-β-<scp>D</scp>-glucanase Gene (<i>neg-1</i>) from<i>Neurospora crassa</i>

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Abstract

The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.

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