A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism in Arabidopsis
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- SAITO Shigeki
- Institute for Chemical Research, Kyoto University
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- OKAMOTO Masanori
- Plant Science Center, RIKEN Department of Biological Sciences, Tokyo Metropolitan University
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- SHINODA Shoko
- Plant Science Center, RIKEN
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- KUSHIRO Tetsuo
- Plant Science Center, RIKEN
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- KOSHIBA Tomokazu
- Department of Biological Sciences, Tokyo Metropolitan University
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- KAMIYA Yuji
- Plant Science Center, RIKEN
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- HIRAI Nobuhiro
- International Innovation Center, Kyoto University
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- TODOROKI Yasushi
- Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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- SAKATA Kanzo
- Institute for Chemical Research, Kyoto University
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- NAMBARA Eiji
- Plant Science Center, RIKEN
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- MIZUTANI Masaharu
- Institute for Chemical Research, Kyoto University
Bibliographic Information
- Other Title
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- Plant Growth Retardant Uniconazole Is a Potent Inhibitor of ABA Catabolism in Arabidopsis
- A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism in<i>Arabidopsis</i>
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Abstract
Plant growth retardants (PGRs) reduce the shoot growth of plants by inhibiting gibberellin biosynthesis. In this study, we performed detailed analyses of the inhibitory effects of PGRs on Arabidopsis abscisic acid (ABA) 8′-hydroxylase, a major ABA catabolic enzyme, recently identified as CYP707As. In an in vitro assay with CYP707A3 microsomes expressed in insect cells, uniconazole-P inhibited CYP707A3 activity more effectively than paclobutrazol or tetcyclacis, whereas the other PGRs tested did not inhibit it significantly. Uniconazole-P was found to be a strong competitive inhibitor (Ki=8.0 nM) of ABA 8′-hydroxylase. Uniconazole-P-treated Arabidopsis plants showed enhanced drought tolerance. In uniconazole-P-treated plants, endogenous ABA levels increased 2-fold as compared with the control, and co-application of GA4 did not suppress the effects, indicating that the effects were not due to gibberellin deficiency. Thus uniconazole-P effectively inhibits ABA catabolism in Arabidopsis plants. We also discuss the structure-activity relationship of the azole-type compounds on ABA 8′-hydroxylase inhibitory activity.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (7), 1731-1739, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206479849984
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- NII Article ID
- 10018529395
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7998063
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed