Synthesis of Stable Isotope-Labeled Precursors for the Biosyntheses of Capsaicinoids, Capsinoids, and Capsiconinoids
-
- KOBATA Kenji
- Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka Faculty of Pharmaceutical Sciences, Josai University
-
- MIMURA Makoto
- Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka
-
- SUGAWARA Mai
- Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka
-
- WATANABE Tatsuo
- Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka
Search this article
Abstract
Stable isotope-labeled precursors were synthesized for an analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to elucidate the biosynthetic flow of capsaicinoids, capsinoids, and capsiconinoids. [1′-13C][5-2H]-Vanillin was prepared by the condensation of guaiacol with [13C]-chloroform and a D2O treatment. Labeled vanillylamine, vanillyl alcohol, ferulic acid, and coniferyl alcohol were prepared from the labeled vanillin. The labeled vanillylamine was converted to labeled capsaicinoid in a crude enzyme solution extracted from pungent Capsicum fruits.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 75 (8), 1611-1614, 2011
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206479878272
-
- NII Article ID
- 10029591963
-
- NII Book ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 11222633
-
- PubMed
- 21821932
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed