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Characterization of Phospholipids in Membrane Vesicles Derived from Pseudomonas aeruginosa

  • TASHIRO Yosuke
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • INAGAKI Aya
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • SHIMIZU Motoyuki
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • ICHIKAWA Sosaku
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • TAKAYA Naoki
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • NAKAJIMA-KAMBE Toshiaki
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • UCHIYAMA Hiroo
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • NOMURA Nobuhiko
    Graduate School of Life and Environmental Sciences, University of Tsukuba

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Abstract

Many Gram-negative bacteria release membrane vesicles (MVs), but their phospholipid properties are poorly understood. Phosphatidylglycerol was present at high levels in MVs derived from Pseudomonas aeruginosa, but not in the cellular outer membrane. The ratio of stearic acid in MVs was high compared to that in the cellular outer membrane. These findings suggest that membrane rigidity is associated with MV biogenesis.

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