Comparison of the Enzymatic Properties of<i>ent</i>-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
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- HAYASHI Yutaka
- Biotechnology Research Center, Toyama Prefectural University
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- TOYOMASU Tomonobu
- Department of Bioresource Engineering, Yamagata University
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- HIROSE Yuko
- Department of Bioresource Engineering, Yamagata University
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- ONODERA Yu
- Department of Bioresource Engineering, Yamagata University
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- MITSUHASHI Wataru
- Department of Bioresource Engineering, Yamagata University
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- YAMANE Hisakazu
- Biotechnology Research Center, University of Tokyo
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- SASSA Takeshi
- Department of Bioresource Engineering, Yamagata University
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- DAIRI Tohru
- Biotechnology Research Center, Toyama Prefectural University
書誌事項
- タイトル別名
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- Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
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説明
The rice genome contains two ent-copalyl diphosphate synthase genes: OsCPS1 acts in gibberellin (phytohormone) biosynthesis, and OsCPS2/OsCyc2 acts in the synthesis of oryzalexins A-F and phytocassanes A-E (phytoalexins). We characterized the enzymatic properties of recombinant OsCPS2/OsCyc2 fused with a tag-protein at the N-terminus, and compared them to those of OsCPS1. Several enzymatic properties of OsCPS2/OsCyc2, including the optimal pH, optimal temperature, divalent cation requirement, and kinetic values for the geranylgeranyl diphosphate (GGDP) substrate, were almost the same as those of OsCPS1. However, OsCPS2/OsCyc2 activity was not inhibited by 50–60 μM GGDP substrate, by which the OsCPS1 activity was inhibited. Furthermore, the OsCPS1 activity exhibited approximately 70% inhibition by 100 μM Amo-1618 (a gibberellin biosynthetic inhibitor), whereas the OsCPS2/OsCyc2 activity exhibited approximately 10% inhibition. These results indicate that the properties of OsCPS2/OsCyc2 were partially different from those of OsCPS1, although OsCPS2/OsCyc2 catalyzes the same reaction step as OsCPS1.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (2), 523-530, 2008
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206480089856
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- NII論文ID
- 10027524584
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 9410267
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- PubMed
- 18256497
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- 本文言語コード
- en
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