Cloning of the Pyridoxine 5′-Phosphate Phosphatase Gene (<i>pdxP</i>) and Vitamin B<sub>6</sub>Production in<i>pdxP</i>Recombinant<i>Sinorhizobium meliloti</i>
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- NAGAHASHI Yoshie
- Department of Applied Microbiology, Nippon Roche Research Center
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- TAZOE Masaaki
- Department of Applied Microbiology, Nippon Roche Research Center
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- HOSHINO Tatsuo
- Department of Applied Microbiology, Nippon Roche Research Center
書誌事項
- タイトル別名
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- Cloning of the Pyridoxine 5'-Phosphate Phosphatase Gene (pdxP) and Vitamin B6 Production in pdxP Recombinant Sinorhizobium meliloti
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説明
A novel gene (pdxP) encoding a pyridoxine 5′-phosphate (PNP) phosphatase involved in the last step of pyridoxine biosynthesis was cloned from Sinorhizobium meliloti IFO 14782 on the basis of the peptide sequences of the natural enzyme. The pdxP gene is an open reading frame (708 bp) encoding 235 amino acid residues with a calculated molecular weight of 26,466. From its deduced amino acid sequence, it was predicted that the enzyme belongs to the haloacid dehalogenase superfamily.<BR>Transformants of Escherichia coli and S. meliloti by pdxP gene expression plasmids showed stimulated PNP phosphatase activities. When pdxP was overexpressed together with the PNP synthase gene (pdxJ) in S. meliloti, the recombinant strain produced 149 mg/l of pyridoxine, 46% and 16% higher than the host strain and the pdxJ recombinant of S. meliloti respectively.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (2), 421-427, 2008
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206480115584
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- NII論文ID
- 10027524261
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 9410125
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- 本文言語コード
- en
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