Purification and Characterization of a Major Collagenase from<i>Streptomyces parvulus</i>

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  • SAKURAI Yasuko
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo
  • INOUE Hideshi
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo School of Life Sciences, Tokyo University of Pharmacy and Life Sciences
  • NISHII Wataru
    School of Life Sciences, Tokyo University of Pharmacy and Life Sciences
  • TAKAHASHI Takayuki
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo
  • IINO Yuichi
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo
  • YAMAMOTO Masayuki
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo
  • TAKAHASHI Kenji
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo School of Life Sciences, Tokyo University of Pharmacy and Life Sciences

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  • Purification and Characterization of a Major Collagenase from Streptomyces parvulus

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A major collagenase was purified about 96-fold from a crude enzyme sample of Streptomyces parvulus by chromatography on Q-Sepharose, Sephacryl S-200, and butyl-Toyopearl. The purified enzyme showed a relative molecular mass of approximately 52,000 on SDS–PAGE and a pH optimum at about 9.0, and was strongly inhibited by metal-chelating agents. It also cleaved 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg specifically at the Leu-Gly bond, with a Km value of 0.60 mM at pH 9.0 at 37 °C. Based on the amino acid sequences of the N-terminal region and internal tryptic peptides, the corresponding gene was cloned. The DNA sequence of the cloned gene indicated that the enzyme is produced as an 864-residue precursor protein with a 408-residue prepro sequence followed by a 456-residue mature enzyme moiety. The enzyme is most homologous with the collagenase from S. coelicolor, the identity being 73%, and it is thought to be a member of the Vibrio collagenase subfamily.

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