Deciphering the Roles of Glycan Processing in Glycoprotein Quality Control through Organic Synthesis
-
- ITO Yukishige
- Synthetic Cellular Chemistry Laboratory, RIKEN ERATO, Japan Science and Technology Agency (JST), Glycotrilogy Project
-
- TAKEDA Yoichi
- ERATO, Japan Science and Technology Agency (JST), Glycotrilogy Project
Bibliographic Information
- Other Title
-
- Award Review : Deciphering the Roles of Glycan Processing in Glycoprotein Quality Control through Organic Synthesis
Search this article
Abstract
Protein quality control (QC) in the endoplasmic reticulum (ER) comprises many aspects, including folding and transport of nascent proteins and degradation of misfolded proteins. Recent studies have revealed that high-mannose-type glycans play pivotal roles in the QC process. To gain knowledge of the molecular basis of this process with well-defined homogeneous compounds, we achieved a convergent synthesis of high-mannose-type glycans and their functionalized derivatives. A major part of our study focused on analyses of UDP-Glc: glycoprotein glucosyltransferase (UGGT) and ER glucosidase II, which play crucial roles in glycoprotein QC, to clarify their specificities. In addition, we established an in vitro assay system mimicking the in vivo condition, which is highly crowded due to the presence of various macromolecules.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 77 (12), 2331-2338, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206480258432
-
- NII Article ID
- 130003381969
-
- NII Book ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BC2c3ks1aksQ%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 025124017
-
- PubMed
- 24317068
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed