Efficient Biotransformations Using<i>Escherichia coli</i>with<i>tolC</i><i>acrAB</i>Mutations Expressing Cytochrome P450 Genes
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- FUJII Tadashi
- Bioresource Laboratories, Mercian Corporation
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- FUJII Yoshikazu
- Bioresource Laboratories, Mercian Corporation
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- MACHIDA Kazuhiro
- Bioresource Laboratories, Mercian Corporation
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- OCHIAI Atsushi
- Bioresource Laboratories, Mercian Corporation
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- ITO Masashi
- Bioresource Laboratories, Mercian Corporation
書誌事項
- タイトル別名
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- Efficient Biotransformations Using Escherichia coli with tolC acrAB Mutations Expressing Cytochrome P450 Genes
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抄録
We report here some efficient biotransformations using Escherichia coli strains with disruptions for the AcrAB-TolC efflux pump system. Biotransformations of compactin into pravastatin (6α-hydroxy-iso-compactin) were performed using E. coli strains with tolC and/or acrAB mutations expressing a cytochrome P450 (P450) gene. The production levels of pravastatin using strains with acrAB, tolC, and tolC acrAB mutations increased by 3.7-, 7.0-, and 7.1-fold, respectively. Likewise, the production levels of 25-hydroxy vitamin D3 and 25-hydroxy 4-cholesten 3-one using tolC acrAB mutant strains expressing an individual P450 gene increased by 2.2- and 16-fold, respectively. The enhancement of this biotransformation efficiency could be explained by increases in the intracellular amounts of substrates and the concentrations of active P450s. These results demonstrate that we have achieved versatile methods for efficient biotransformations using E. coli strains with tolC acrAB mutations expressing P450 genes.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 73 (4), 805-810, 2009
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206480354560
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- NII論文ID
- 10027539458
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10212702
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可