Cloning and Expression of a Novel Sulfotransferase with Unique Substrate Specificity from<i>Bombyx mori</i>
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- HATTORI Kenji
- Kyoritsu University of Pharmacy
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- HIRAYAMA Mio
- Kyoritsu University of Pharmacy
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- SUZUKI Hiroko
- Kyoritsu University of Pharmacy
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- HAMAMOTO Hiroshi
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- SEKIMIZU Kazuhisa
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- TAMURA Hiro-omi
- Kyoritsu University of Pharmacy
書誌事項
- タイトル別名
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- Cloning and Expression of a Novel Sulfotransferase with Unique Substrate Specificity from Bombyx mori
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We identified a cDNA encoding a putative cytosolic sulfotransferase (SULT) by searching the expressed sequence tag database of Bombyx mori, and subsequently obtained the full-length cDNA for this gene via rapid amplification of cDNA ends (RACE). We designated this gene bmST1, and showed by sequence analysis that it belongs to a novel SULT family. The tissue specificity of bmST1 mRNA expression was examined in fifth instar larvae by reverse transcriptase-polymerase chain reaction (RT-PCR), and transcripts were detectable in the silk gland, gut, fat body, and Malpighian tube. A recombinant form of bmST1 was then expressed using a gluthathione S-transferase (GST) gene fusion system, and it was purified from Escherichia coli. Purified bmST1 did not exhibit sulfating activity toward SULT substrates such as 4-nitrophenol, vanillin, hydroxysteroids, or monoamines. Surprisingly, however, recombinant bmST1 showed considerable activity toward 4-nitrocatechol and also gallate esters, although the catechins are not sulfated by this enzyme.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (4), 1044-1051, 2007
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206480390144
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- NII論文ID
- 10027513902
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8715304
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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