Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine N-Methyltransferase Gene

DOI Web Site Web Site 6 Citations 30 References
  • TABUCHI Tomoki
    Laboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University
  • OKADA Tomoyuki
    Laboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University
  • AZUMA Tetsushi
    Graduate School of Science and Technology, Kobe University
  • NANMORI Takashi
    Graduate School of Science and Technology, Kobe University
  • YASUDA Takeshi
    Laboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University Graduate School of Science and Technology, Kobe University

Bibliographic Information

Other Title
  • Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine<i>N</i>-Methyltransferase Gene

Search this article

Abstract

Phosphoethanolamine N-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5′ untranslated region (UTR) of several PEAMT genes was found to contain an upstream open reading frame (uORF). We generated transgenic Arabidopsis calli that expressed a chimeric gene constructed by fusing the 5′ UTR of the Arabidopsis PEAMT gene (AtNMT1) upstream of the β-glucuronidase gene. The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream β-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.

Journal

Citations (6)*help

See more

References(30)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top