FAB CID-MS/MS of In-Source Fragment Ions as a Means to Differentiate Linkage Isomers of Branched Oligosaccharides: Lea and Lex Type Glycoconjugates.
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- OHASHI Yoko
- Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research
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- II Tadashi
- Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research
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- KUBOTA Masayuki
- Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research Department of Applied Physics and Chemistry, The University of Electro-Communications
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- NUNOMURA Shigeki
- Tokyo Pharmaceutical Research Laboratories, Nissin Food Products Co., Ltd.
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- NIWA Haruki
- Department of Applied Physics and Chemistry, The University of Electro-Communications
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- OHASHI Mamoru
- Department of Applied Physics and Chemistry, The University of Electro-Communications
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- OGAWA Tomoya
- Laboratory of Synthetic Cellular Chemistry, The Institute of Physical and Chemical Research Faculty of Agriculture, University of Tokyo
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- NAGAI Yoshitaka
- Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research Mitsubishikasei Institute of Life Sciences
Bibliographic Information
- Other Title
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- FAB CID-MS MS of In-Source Fragment Ion
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Abstract
Here we report that FAB CID-MS/MS, choosing an ion originating from the in-source degradation prior to the full acceleration of the molecule-related ion as the precursor, is an excellent means to differentiate linkage isomers. FAB CID-MS/MS performed in this way results in a product ion spectrum, which is enriched with the fragmentation information, and which may thus indicate the position of the newly formed OH group already produced in the ion source. Linkage knowledge may be classified into one of three categories as described later. This method was applied to acidic Lewis-type glycoconjugates —putative ligands for selectins [L. L. W. Cooling, D.-S. Zhang, and T. A. W. Koerner, Trends in Glycosci. Glycotech., 9, 191 (1997)]— and their neutral analogs to differentiate linkage isomers by mass spectrometry. With these glycoconjugates FAB CID-MS/MS having [M+H]+s as the precursor ions is a good means to distinguish the linkage isomers only in simple cases, but not in general cases. By using ions originating from in-source degradation prior to the full acceleration of the molecule-related ion as the precursor, we were able to differentiate between Lea and Lex.
Journal
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- Journal of the Mass Spectrometry Society of Japan
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Journal of the Mass Spectrometry Society of Japan 46 (1), 45-52, 1998
The Mass Spectrometry Society of Japan
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Details 詳細情報について
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- CRID
- 1390001206494419200
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- NII Article ID
- 10016281933
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- NII Book ID
- AN0010555X
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- COI
- 1:CAS:528:DyaK1cXhs1ahtrs%3D
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- ISSN
- 18804225
- 18843271
- 13408097
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- NDL BIB ID
- 4402992
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed