Utility of Application of Liquid Chromatography/Triple Stage Mass Spectrometry (LC/MS3) for Proteome Analysis
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- FUJII Kiyonaga
- Graduate School of Pharmaceutical Sciences, Hokkaido University
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- NAKANO Tomoyo
- AMR, Inc.
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- USUI Fumihiko
- AMR, Inc.
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- BANDO Yasuhiko
- AMR, Inc.
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- INAGAKI Fuyuhiko
- Graduate School of Pharmaceutical Sciences, Hokkaido University
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- NISHIMURA Toshihide
- Department of Surgery I, Tokyo Medical University
Bibliographic Information
- Other Title
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- プロテオーム解析における液体クロマトグラフィー3段階質量分析法(LC/MS`3´) の有用性について
- プロテオーム解析における液体クロマトグラフィー3段階質量分析法(LC/MS[3]) の有用性について
- プロテオーム カイセキ ニ オケル エキタイ クロマトグラフィー 3 ダンカイ シツリョウ ブンセキホウ LC MS3 ノ ユウヨウセイ ニ ツイテ
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Abstract
Protein identification via mass spectrometry (MS) relies primarily on protein sequence databases. Identification resulting from a sequence database search in the tandem mass spectrometry (MS/MS) experiments for the digested peptides is derived from an algorithm based on statistical confidence, and therefore, it often pro- ides false-positive identifications. Although one can apply high-resolution MS such as Fourier-transform mass spectrometer (FT-MS) to obtain results with high confidence, the instrumentation is expensive and the results are still probability based. Therefore, we introduced data-dependent triple stage mass spectrometry (MS3) into our well-established liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis of linear ion trap MS to achieve evidence-based protein identification. Peptide sequencing by MS3 analysis can provide a direct evidence for a sequence elucidated by MS/MS data and help determine whether the search results are correct.
Journal
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- Journal of the Mass Spectrometry Society of Japan
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Journal of the Mass Spectrometry Society of Japan 57 (4), 317-325, 2009
The Mass Spectrometry Society of Japan
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Details 詳細情報について
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- CRID
- 1390001206495166848
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- NII Article ID
- 10025571784
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- NII Book ID
- AN0010555X
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- ISSN
- 18804225
- 18843271
- 13408097
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- NDL BIB ID
- 10324162
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed