Discrimination of living or dead cells of diatom based on the stained images - Its method and significance.

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  • KOSUGI Masato
    Graduate School, Department of Applied Earth Sciences, Nihon University

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  • 染色像によるけいそうの生体・遺がいの識別法とその意義
  • センショクゾウ ニ ヨル ケイソウ ノ セイタイ イガイ ノ シキベツホウ ト
  • Its Method and Significance

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It is an important and fundamental study in paleoecology to examine accurately the distribution and ecology of living assemblages. In the case of diatoms, however, living assemblages have been unalbe to be selected from the total assemblages including dead cells.<br>Hematoxylin and Eosin stain which is in general use to observe cell contents of organisms anatomically is used in this study to distinguish between living and dead cells of diatoms. Moreover, characteristics in the distribution of diatoms obtained from the lower reach of the Obitsu river, Chiba Prefecture, central Japan, are investigated by this method.<br>The results are as follows;<br>1. The images of nucleus dyed by Hematoxylin and the part of cytoplasm, considered to be chromatophore, dyed by Eosin show patterns peculiar to some groups of species. The dyed images are classified into four types on the basis of characteristics in their shape, position (nucleus and cytoplasm) and number (cytoplasm). Only completely dyed cells are identified as living, and partly-dyed cells and undyed cells as dead.<br>2. In the Obitsu river, living assemblages peculiar to such environments as tidal flat, tidal inlet, salt pond and estuary were recognized as shown in Fig. 2a. These environments are characterized by differences in salinity, bottom sediment, accumulation of organic matter and current of water. On the other hand, dead cells were diffused to the outside of the living area (Fig. 2b). In order to analyze fossil assemblages, it is necessary to make use of the data obtained from present environments.

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