A Processing Technique for Cell Surfaces Using Gas Cluster Ions for Imaging Mass Spectrometry

DOI Web Site Web Site 参考文献34件
  • YAMADA Hideaki
    Department of Nuclear Engineering, Kyoto University
  • ICHIKI Kazuya
    Department of Nuclear Engineering, Kyoto University
  • NAKATA Yoshihiko
    Department of Nuclear Engineering, Kyoto University
  • NINOMIYA Satoshi
    Quantum Science and Engineering Center, Kyoto University
  • SEKI Toshio
    Department of Nuclear Engineering, Kyoto University CREST, Japan Science and Technology Agency
  • AOKI Takaaki
    Department of Electronic Science, and Engineering, Kyoto University CREST, Japan Science and Technology Agency
  • MATSUO Jiro
    Quantum Science and Engineering Center, Kyoto University CREST, Japan Science and Technology Agency

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抄録

We developed a processing technique for biological samples, that is, animal cells, for imaging mass spectrometry using gas cluster ion beams. Secondary ion mass spectrometry has been investigated for cellular imaging with high spatial resolution. Nanoscale processing techniques are needed for cellular level imaging, for example, for removing contamination from cell surfaces and exposing the insides of cells. In this study, we compared the mass spectrum of the unetched cell sample to that of the cell irradiated with an Ar cluster ion beam. The contamination was removed from the sample surface and the signals of intracellular components were detected. The results indicate that the cells were etched with low damage using the gas cluster ion beam. Finally, we compared the ion image of an unetched cell to that of an etched cell and demonstrated that the technique of cluster ion irradiation could be applied to the processing of samples for cellular level imaging mass spectrometry.

収録刊行物

  • 質量分析

    質量分析 57 (3), 117-121, 2009

    一般社団法人 日本質量分析学会

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