Bioelectrocatalytic Reduction of Oxygen by Hemoglobin-Adsorbed Carbon-Felt, and its Inhibition by Azide

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  • HASEBE Yasushi
    Department of Life Science and Green Chemistry, Saitama Institute of Technology
  • WANG Yue
    School of Chemical Engineering, University of Science and Technology LiaoNing

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Abstract

Hemoglobin (Hb) was adsorbed onto a carbon-felt (CF), which is a microelectrode ensemble of microcarbon fiber (ca. 7 µm diameter) and possesses a random three-dimensional structure. Direct electron transfer between Hb heme and the CF was achieved without any electron mediating species and special materials. The Hb-adsorbed CF (Hb-CF) showed a pair of well-defined cyclic voltammetric peaks with the formal potential of −0.221 V (vs. Ag/AgCl) at pH 5.0 (0.1 M phosphate/citrate buffer), which is attributed to Hb Fe(III)/Fe(II) redox couple. The apparent heterogeneous electron-transfer rate constant (ks) was estimated to be 14.6 s−1. Furthermore, the Hb-CF exhibited an excellent bioelectrocatalytic activity for the reduction of O2. This bioelectrocatalytic activity was inhibited by azide, which binds to active heme center of Hb.

Journal

  • Electrochemistry

    Electrochemistry 80 (5), 358-362, 2012

    The Electrochemical Society of Japan

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