Nitrous Oxide Sensing using Oxygen-Insensitive Direct-Electron-Transfer-Type Nitrous Oxide Reductase

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  • TSUGAWA Wakako
    Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology
  • SHIMIZU Hitomi
    Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology
  • TATARA Masahiro
    Kajima Technical Research Institute
  • UENO Yoshiyuki
    Kajima Technical Research Institute
  • KOJIMA Katsuhiro
    Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology
  • SODE Koji
    Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology

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Abstract

Nitrous oxide (N2O) is known as a greenhouse gas and a dominant ozone-depleting substance. It is is released mainly from agricultural processes. Therefore, the development of an on-site monitoring system is required to measure N2O concentration and control the release from the source. Using oxygen-insensitive cytochrome c-type nitrous oxide reductase, wNosZ, from Wolinella succinogenes, we have developed an electrochemical enzyme sensor. The sensor signal depends on the concentration of N2O in a reaction cell containing methoxy-5-methylphenazinium methylsulfate (mPMS) as an electron mediator under Ar atmosphere and, surprisingly, under air. Moreover, in the absence of addition of an electron mediator to the reaction cell, we observe that the reduction current depends on the concentration of N2O, which implies direct electron transfer. The wNosZ electrode is stable when stored at 4°C for 2 weeks and is specific to N2O. These results suggest that wNosZ holds great promise as a component of a novel direct-electron-transfer-type electrochemical sensing system for N2O.

Journal

  • Electrochemistry

    Electrochemistry 80 (5), 371-374, 2012

    The Electrochemical Society of Japan

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