New Immunosensor by Latex Adhesion Column Method

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  • SUEMITSU Junsuke
    Department of Material Systems Engineering, Faculty of Technology, Tokyo University
  • SAKURAI Satoshi
    Department of Material Systems Engineering, Faculty of Technology, Tokyo University
  • OGAWA Kimihiro
    Department of Material Systems Engineering, Faculty of Technology, Tokyo University
  • AKAIKE Toshihiro
    Department of Material Systems Engineering, Faculty of Technology, Tokyo University

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Other Title
  • 高分子マイクロスフィアを利用した免疫センサシステム
  • コウブンシ マイクロスフィア オ リヨウシタ メンエキ センサ システム

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Abstract

Antigen-antibody reactions on polymer surface were examined by thc use of newly developed “Latcx Adhesion Column Method”and the availability of this method for the immunosensor was evaluated. This method was classified to two types, the direct method and the indirect method. In the direct method, microspheres (MS) were coated with antigen and latices were coated with antibody, and direct binding reactions between antigen-coatod MS and antibody-coated latices were estimated. In the indirect method, both of MS and latices were coated with the same antibody and thereafter the examined antigen was used in the medium. Such sandwitch type of reaction as antibody-antigen-antibody trimolecular complex formation was investigated. In our study, polystyrene was adopted as polymeric material, and rheumatoid factor (Rf), fibrinogen degradation product D (FDP-D), and human IgG were selected as the antigens. The results were as follows. (1) Difference of latices adhesion behaviors among three types was observed, which might correspond to adsorption mechanism and the amount of antigen. (2) In autoimmune disease dystem such as Rf system, the effect of inhibition of latices adhesion was observed possibly because of the influence of adsorbed denatured γ-G in the serum of the patient. (3) In some cases, “Latex Adhesion Column Method”was turned out to be very available for the immunosensor.

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 41 (4), 249-256, 1984

    The Society of Polymer Science, Japan

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