Supercritical Fluid in Polymer Science and Technology. I. Reversible Activity Control of Enzymatic Reactions in Supercritical Fluid. Enantioselective Esterification Catalyzed by a Lipid-Coated Lipase in Supercritical Fluoroform.

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  • MORI Toshiaki
    Department of Biomolecular Engineering, Tokyo Institute of Technology
  • FUNASAKI Mariko
    Department of Biomolecular Engineering, Tokyo Institute of Technology
  • KOBAYASHI Atsushi
    Department of Biomolecular Engineering, Tokyo Institute of Technology
  • OKAHATA Yoshio
    Department of Biomolecular Engineering, Tokyo Institute of Technology

Bibliographic Information

Other Title
  • 超臨界流体と高分子　Ｉ　　超臨界流体中での酵素反応の制御　　超臨界フルオロフォルム中での脂質修飾リパーゼを用いた不斉選択的エステル化反応
  • チョウリンカイ リュウタイ チュウ デ ノ コウソ ハンノウ ノ セイギョ チョウリンカイ フルオロフォルム チュウ デ ノ シシツ シュウショク リパーゼ オ モチイタ フセイ センタクテキ エステルカ ハンノウ
  • Enantioselective Esterification Catalyzed by a Lipid Coated Lipase in Supercritical Fluoroform
  • 超臨界フルオロフォルム中での脂質修飾リパーゼを用いた不斉選択的エステル化反応

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Description

The lipid-coated lipase is soluble and can catalyze esterification enantioselectively in supercritical fluoroform (scCHF3), as well as organic solvents. In organic media, the lipid-coated lipase became inactive in polar solvents due to denaturation of enzymes. One of the features of supercritical fluid is that physical properties such as polarity or dielectric constant could be reversibly controlled by changing pressure or temperature of scCHF3. When the lipid-coated lipase was employed in scCHF3 by changing pressure or temperature, the reaction rate could be regulated and the conversion was kept constant in high yields. Thus, the enzyme activity could switch on and off by adjusting pressure or temperature of scCHF3. We believe that the combination of the lipid-coated enzyme and scCHF3 as reaction media will become a new system as biotransformation studies.

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 58 (10), 564-568, 2001

    The Society of Polymer Science, Japan

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