Basic Characteristics of Cellulase Immobilized on Lignophenol

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  • リグノフェノールを担体とする固定化セルラーゼの基礎的特性
  • リグノフェノール オ タンタイ ト スル コテイカ セルラーゼ ノ キソテキ トクセイ

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Abstract

Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable.<br>

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 68 (5), 315-319, 2011

    The Society of Polymer Science, Japan

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