Preparation of Purified Natural Rubber by Removal of Proteins

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  • FUKUHARA Lina
    Tokyo National College of Technology, Department of Chemical Science and Engineering
  • MIYANO Kai
    Tokyo National College of Technology, Department of Chemical Science and Engineering
  • YAMAMOTO Yoshimasa
    Tokyo National College of Technology, Department of Chemical Science and Engineering
  • ISHII Hiroyuki
    Tokyo National College of Technology, Department of Chemical Science and Engineering
  • KAWAHARA Seiichi
    Department of Materials Science and Technology, Faculty of Engineering, Nagaoka University of Technology

Bibliographic Information

Other Title
  • タンパク質を除去することによる精製天然ゴムの調製
  • タンパクシツ オ ジョキョ スル コトニ ヨル セイセイ テンネン ゴム ノ チョウセイ

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Abstract

An attempt was made to prepare purified natural rubber by removal of proteins to achieve a low extractable protein content. The removal of the proteins were performed by leaching natural rubber gloves possessing high extractable protein content and high nitrogen content and by deproteinization of high ammonia natural rubber latex with urea, sodium dodecyl sulfate (SDS) and acetone. The extractable protein content and nitrogen content of the purified natural rubber were measured by the modified Lowry method and the Kjeldahl method. The extractable protein content of the natural rubber glove was reduced from 692 µg/g to 86 µg/g by leaching, while the nitrogen content decreased a little. The nanomatrix of non-rubber content of the natural rubber glove disappeared after leaching at 60, 80 and 100°C, as observed with a focused ion beam / scanning electron microscope. In contrast, the extractable protein content and the nitrogen content of natural rubber were reduced to 0.0 µg/g and 0.000 w/w%, after deproteinization with urea, SDS and acetone.

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 72 (1), 1-6, 2015

    The Society of Polymer Science, Japan

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