Molecular Characterization of Water Soluble Polymers by Field-Flow Fractionation Multiangle Light Scattering FFF-MALS

DOI Web Site 1 Citations 41 References
  • HIRAMATSU Tsuyoshi
    Department of Polymer Science and Engineering, Graduate School of Science and Engineering, Yamagata University
  • OHNO Satoshi
    Department of Polymer Science and Engineering, Graduate School of Science and Engineering, Yamagata University
  • SAITO Yuta
    Department of Polymer Science and Engineering, Graduate School of Science and Engineering, Yamagata University
  • TOGASHI Daichi
    Department of Polymer Science and Engineering, Graduate School of Science and Engineering, Yamagata University
  • KAWAGUCHI Seigou
    Department of Polymer Science and Engineering, Graduate School of Science and Engineering, Yamagata University

Bibliographic Information

Other Title
  • Field-Flow Fractionation-MALSを用いた水溶性高分子の分子特性解析
  • Field-Flow Fractionation : MALS オ モチイタ スイヨウセイ コウブンシ ノ ブンシ トクセイ カイセキ

Search this article

Abstract

Field-flow fractionation (FFF) is one of the promising alternative separation techniques to size exclusion chromatography (SEC). In this study, water soluble polymers including Bovine Serum Albumin (BSA), pullulan, poly(ethylene oxide) (PEO), sodium poly(styrene sulfonate) (NaPSS), and partially saponified poly(vinyl alcohol) (PVA) have successfully been characterized by a means of the FFF technique, fitted with a multiangle laser light scattering (MALS) detector in 0.1 M Na NO3 as an eluent at 25°C. The factors which affect the retention time, tr, and the separation factor, Rs, of the monomer and dimer of BSA are the thickness of spacer, w, channel flow rate, VCHF, cross-flow rate, VCRF, focusing time, injection volume, and polymer concentration, Cp. We found that the retention time, tr, in the FFF follows the equation of tr~[VCHF]−1.0[VCRF]1.0[w]2.0, in agreement with theory. Several cross-flow rates were examined for the characterization of the water soluble flexible polymers and the polyelectrolyte, and an appropriate gradient method for these polymers is found to be described by the equation of VCRF = A exp (−Btr), where A and B are constant. For the polyelectrolyte, the retention time significantly varies with salt concentration, due to the balance between change in the conformation of polyelectrolyte chains and the electrostatic exclusion from the membrane filter.

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 70 (2), 72-81, 2013

    The Society of Polymer Science, Japan

Citations (1)*help

See more

References(41)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top