歯髄細胞を組み込んだPLGA神経誘導管は顔面神経再生を促進する

  • 佐々木 亮
    東京女子医科大学医学部歯科口腔外科学教室 東京女子医科大学先端生命医科学研究所
  • 内山 博人
    東京女子医科大学医学部歯科口腔外科学教室
  • 扇内 秀樹
    東京女子医科大学医学部歯科口腔外科学教室
  • 安藤 智博
    東京女子医科大学医学部歯科口腔外科学教室

書誌事項

タイトル別名
  • PLGA artificial nerve conduits with dental pulp cells promote facial nerve regeneration
  • シズイ サイボウ オ クミコンダ PLGA シンケイ ユウドウカン ワ ガンメン シンケイ サイセイ オ ソクシン スル

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説明

A number of recent studies have shown the effectiveness of tubulation, using neural progenitor cells or Schwann cells, for promoting nerve regeneration. However, the use of neural cells from other neural donor tissues can cause potentially serious clinical complications. Therefore, we focused on dental pulp as a new cell source for use in such artificial conditions. Previously, we showed that silicone tubes filled with dental pulp cells (DPCs) promoted facial nerve regeneration in rats. However, the use of silicone tubes requires a secondary removal operation because they may give rise to chronic inflammation and pain. Therefore, to avoid this procedure, a new artificial device was prepared from a degradable Copoly-lactide/glycolide (PLGA) tube containing DPCs, and its effectiveness for repairing gaps in the facial nerves of rats was investigated. A PLGA tube containing rat DPCs embedded in collagen gel was transplanted into a gap in a rat facial nerve. Five days after transplantation, the facial nerves connected by the PLGA tubes containing DPCs were repaired more quickly than the control nerves. The PLGA tubes were resorbed in vivo, and nerve regeneration was observed 2 months after transplantation. Immunostaining showed that Tuj1-positive axons were present in the regenerated nerves 2 months after transplantation, and osmium-toluidine blue staining showed no mineralization of the regenerated nerves in tubes containing myelinated fibers after 9 weeks. PLGA tubes filled with DPCs promoted nerve regeneration and were readily resorbed in vivo.

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