ミオシン首振り説: 部位特異的変異による検証から構造遺伝学によるメカニズム解明へ

  • 上田 太郎
    工業技術院産業技術融合領域研究所バイオニックデザイングループ
  • Patterson R. Bruce
    Molecular and Cellular Biology, University of Arizona

書誌事項

タイトル別名
  • Swinging lever arm model for force production by myosin: Testing by site-directed mutant approach and analyses of the mechanism by structural genetics.
  • ミオシン クビフリセツ ブイ トクイテキ ヘンイ ニヨル ケンショウ カラ コ

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抄録

Myosin is a molecular motor that drives movement of actin filaments. It was recently established that the myosin head undergoes a major shape change during the ATP hydrolysis cycle, leading to the popular hypothesis that the shape change provides motile force. Cellular slime mold is uniquely useful not only as an expression system of site-directed mutant myosins, but also as a genetic system to isolate specific mutant myosin genes and their suppressors out of a population of randomly mutagenized cells. Here we present the power of both sitedirected and random approaches to investigate the significance and mechanism of the conformational changes.

収録刊行物

  • 生物物理

    生物物理 37 (1), 331-335, 1997

    一般社団法人 日本生物物理学会

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