Inhibitory effect of plasma on the growth of osteoblastic cells in culture

DOI Web Site 5 Citations 16 References
  • KOUYAMA Naoshi
    First Department of Oral and Maxillofacial Surgery, Meikai University School of Dentistry
  • TAJIMA Masamichi
    Department of Dental Pharmacology, Meikai University School of Dentistry
  • KIKUCHI Hirotaka
    Department of Endodontics, Meikai University School of Dentistry
  • NISHIKAWA Hirofumi
    Department of Endodontics, Meikai University School of Dentistry
  • SAKAGAMI Hiroshi
    Department of Dental Pharmacology, Meikai University School of Dentistry
  • SHIMADA Jun
    First Department of Oral and Maxillofacial Surgery, Meikai University School of Dentistry

Bibliographic Information

Other Title
  • 培養骨芽細胞の増殖に対する血しょうの阻害的作用について
  • バイヨウコツ ガ サイボウ ノ ゾウショク ニ タイスル ケッショウ ノ ソガイテキ サヨウ ニ ツイテ

Search this article

Description

Platelet-rich plasma (PRP), which contains growth factors for osteoblasts, is clinically used to promote regeneration of osseous tissue. However, we found that human plasma dose-dependently inhibits the growth of cultured MC3T3-E1 osteoblastic cells. In this study, the characteristics of osteoblast inhibiting factor in plasma were investigated. The inhibitory effect on MC3T3-E1 cells was inactivated by pre-heating the plasma. The molecular weight of this factor was estimated to be more than 100-kDaltons by the ultrafiltration method, and the macromolecular fraction strongly inhibited the spreading of MC3T3-E1 cells. Moreover, pre-treatment with a serine protease inhibitor abrogated the inhibitory effect of this factor, suggesting that serine proteases have an important role in inhibiting the growth of MC3T3-E1 cells. These results suggest that the improved preparation of platelets to exclude osteoblast-inhibiting factor from PRP is needed for the efficient regeneration of osseous tissue.

Journal

Citations (5)*help

See more

References(16)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top