書誌事項
- タイトル別名
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- <i>In vitro</i> Reactivation of the Contractile Ring in the Fission Yeast Cell-ghost
- 収縮環のin vitro収縮系の開発
- シュウシュクカン ノ in vitro シュウシュクケイ ノ カイハツ
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抄録
Cytokinesis in many eukaryotes involves the closure of an actomyosin-based contractile ring (CR). We have established an in vitro system to study CR contraction. We show that CRs of permeabilized fission yeast cells undergo rapid contraction in an ATP and myosin-II-dependent manner in the absence of other cytoplasmic constituents. Neither actin polymerization nor its disassembly was required for closure of CRs although addition of exogenous actin cross-linking proteins blocked ring contraction. Using CRs generated from fission yeast cytokinesis mutants, we show that not all proteins required for assembly of the CR are required for its contraction in vitro.
収録刊行物
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- 生物物理
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生物物理 54 (4), 201-205, 2014
一般社団法人 日本生物物理学会
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詳細情報 詳細情報について
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- CRID
- 1390001206534702720
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- NII論文ID
- 130004677774
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- NII書誌ID
- AN00129693
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- ISSN
- 13474219
- 05824052
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- NDL書誌ID
- 025757785
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可