Genetic Code Reprogramming

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  • KOJIMA Tatsuya
    Department of Chemistry, Graduate School of Science, The University of Tokyo
  • HAYASHI Yuuki
    Department of Chemistry, Graduate School of Science, The University of Tokyo
  • SUGA Hiroaki
    Department of Chemistry, Graduate School of Science, The University of Tokyo

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Other Title
  • 遺伝暗号リプログラミング技術
  • イデン アンゴウ リプログラミング ギジュツ

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Description

Genetic code reprogramming enables us to assign nonproteinogenic amino acids to codons in place of proteinogenic amino acids according to the genetic code, which allows us to express desired nonstandard peptides using an in vitro translation system. Here, we discuss FIT (Flexible In vitro Translation) system, which is a method that facilitates genetic code reprogramming by employing flexizymes. Furthermore, we also report a method called RaPID (Random non-standard Peptide Integrated Discovery) system, which is an integration of FIT system with an in-vitro mRNA display. RaPID system allows us to select peptides with high binding affinities from highly diverse nonstandard peptide libraries and is quickly becoming a valuable new tool for drug discovery.<br>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 52 (1), 004-009, 2012

    The Biophysical Society of Japan General Incorporated Association

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