Recent Developments in Super Resolution Fluorescence Microscopy
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- FUJITA Katsumasa
- Department of Applied Physics, Osaka University
Bibliographic Information
- Other Title
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- 超解像顕微鏡の進展
- チョウカイゾウ ケンビキョウ ノ シンテン
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Abstract
Recent developments in fluorescence microscopy techniques have broken the diffraction limit and achieved the spatial resolution of sub 100 nm range. Saturated excitation (SAX) microscopy and stimulated emission depletion (STED) microscopy utilize saturable optical phenomena seen in laser excitation and stimulate emission of fluorescence molecules to induce strongly nonlinear optical effects for the resolution improvement. Photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) applied photoswitchable fluorescence probes for precise measurement of positions of the fluorescence probes in a sample in a few tens of nanometer scale. This review introduces the principles and the characteristics of those super resolution microscopy techniques with discussing the imaging formation and the resolution limit in conventional microscopy techniques.<br>
Journal
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- Seibutsu Butsuri
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Seibutsu Butsuri 50 (4), 174-179, 2010
The Biophysical Society of Japan General Incorporated Association
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Keywords
Details 詳細情報について
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- CRID
- 1390001206536192128
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- NII Article ID
- 10026569412
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- NII Book ID
- AN00129693
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- ISSN
- 13474219
- 05824052
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- NDL BIB ID
- 10783897
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed
