Study on Agricultural Utilization of Deproteinized Plant Juice : 1. Suitability of Deproteinized Juice of Alfalfa Plant as Culture Medium for Rhizobilum Species

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  • アルファルファ緑葉脱タンパク上澄液の根粒菌培地としての利用
  • アルファルファ リョクヨウ ダツ タンパク ウワズミエキ ノ コンリュウキン

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In the separation process of leaf protein concentrates (LPC) from alfalfa plant leaves, a large amount of deproteinized juice (alfalfa brown juice : A-BJ) is issued as by-product. The purpose of this investigation is to clarify the chemical composition of A-BJ and to estimate the suitability as culture medium for Rhizobium species. The results were as follows: 1) Values of pH, EC, and dry matter of A-BJ were 5.55, 15.5 mS, and 4.78%, respectively. Three kinds of sugar (glucose, sucrose, and fructose) and 11 kinds of amino acid were contained in A-BJ. Among the amino acids, aspragine was more abundantly contained in A-BJ. 2) The growth of Rhizobium meliloti inoculated in A-BJ medium was higher than that in yeast extract-mannitol (YM) medium. The same tendency was observed on the growth of R. leguminosarum biovar trifolii and R. leguminosarum biovar viceae. On the contrary, the growth of R. leguminosarum biovar phaseoli and B. japonicum was more favorable in diluted A-BJ than in A-BJ and YM media. 3) Rhizobial inoculation resulted in the decrease of glucose, fructose, and sucrose contents and disappearance of asparagine in A-BJ. It was considered from these results that A-BJ was available as a culture medium for Rhizobium species.

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