ゼラチン中のシステイン,ホモシステインとそれらのジスルフィドのHPLCによる定量
書誌事項
- タイトル別名
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- HPLC Determination of Cysteine, Homocysteine and their Disulfides in Gelatin.
説明
The HPLC determination of cysteine, homocysteine and their disulfides in gelatin with isocratic elution and UV detection were investigated. Dithiothreithol (DTT) was used for reduction of disulfides and 4-(aminosulfonyl)-7-fluoro-2, 1, 3-benzoxadiazole (ABD-F), a fluorogenic reagent, was used for precolumn derivatization of thiols. ABD derivatized thiols were eluted isocratically and detected with not only fluorescence but also absorption at 375 nm. The detection limit of the analytes was about 2 ppm in gelatin using UV detection, whereas it was less than 1 ppm using fluorecence detection. The standard deviation was less than 2 ppm at 10 ppm level analysis for both detection methods. One column wash per day was suitable for the proposed conditions.
収録刊行物
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- 日本写真学会誌
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日本写真学会誌 64 (1), 20-24, 2001
社団法人 日本写真学会
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詳細情報 詳細情報について
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- CRID
- 1390001206569790720
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- NII論文ID
- 130004284398
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- ISSN
- 18845932
- 03695662
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可