Viabilities of long-term cryopreserved CHO-TRET1 cells with trehalose and DMSO（CHO-TRET1細胞のトレハロースとDMSOによる凍結保存生存率の保存期間依存性）
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- Tsutomu UCHIDA（内田努）
- Faculty of Engineering, Hokkaido University
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- Maho FURUKAWA（古川真帆）
- Graduate School of Engineering, Hokkaido University
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- Takahiro KIKAWADA（黄川田隆洋）
- Institute of Agrobiological Sciences, National Agriculture and Food Research Organization Graduate School of Frontier Sciences, The University of Tokyo
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- Kenji YAMAZAKI（山崎憲慈）
- Faculty of Engineering, Hokkaido University
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- Kazutoshi GOHARA（郷原一寿）
- Faculty of Engineering, Hokkaido University
書誌事項
- タイトル別名
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- Viabilities of long-term cryopreserved CHO-TRET1 cells with trehalose and DMSO
抄録
<p>Trehalose acts as a non-toxic cryoprotectant agent (CPA) to CHO-TRET1 cells, which are CHO-K1 cells that express the trehalose transporter. This study examines the viabilities during long-term storage at 193K of cryopreserved CHO-TRET1 cells with trehalose, and compares them to the corresponding viabilities with 10wt%-dimethyl sulfoxide (DMSO). The cryopreserved samples are selected to have a 1-week storage viability exceeding 0.7. Results show that this high viability with trehalose is maintained at the same level for about one year of storage, and gradually decrease to about 0.55±0.16 after about 2.5 years. Comparison of this result with the corresponding case with DMSO suggests that the cryoprotective ability of trehalose is slightly weaker than that of DMSO at this temperature. We discuss the effect of recrystallization of ice, and also the biological damaging, on the viabilities. Then we concluded that the cell membrane slowly degrades at 193K, with the rate being a little faster with trehalose than with DMSO.</p>
収録刊行物
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- 氷河情報センター
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氷河情報センター 37 (0), 1-9, 2019
公益社団法人 日本雪氷学会
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詳細情報 詳細情報について
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- CRID
- 1390001277346321536
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- NII論文ID
- 130007702136
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- ISSN
- 18848044
- 13453807
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可