High Resolution Imaging of Bacterial Cell Surface by High Speed Atomic Force Microscopy

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  • Yamashita Hayato
    大阪大学大学院基礎工学研究科附属極限科学センター

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Other Title
  • 高速原子間力顕微鏡によるバクテリア細胞表面の高解像イメージング
  • コウソク ゲンシ カンリョク ケンビキョウ ニ ヨル バクテリア サイボウ ヒョウメン ノ コウカイゾウ イメージング

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<p>Progresses in microscopic techniques have ever contributed to many discoveries in biology. Electron microscopic techniques have revealed that cell membrane structures are organized into functional domains, whereas optical microscopic techniques such as fluorescence imaging have revealed the dynamics of specific molecules in live cell membrane. However, neither technique can directly and simultaneously visualize the structure and dynamics of living cell surface at high spatiotemporal resolution.</p><p>High speed atomic force microscopy (HS-AFM) is powerful tool which visualize the dynamic molecular process in physiological condition. This technique has ever been succeeded in imaging many kinds of proteins purified from cell at high spatiotemporal resolution. However, there are not many applications for cell imaging. In this study, we applied this technique for observing bacterial cell surface. AFM images showed nanostructure and the dynamic processes on bacterial cell outer membrane. Here, we report the result of direct observation on live cell surface using HS-AFM.</p>

Journal

  • KENBIKYO

    KENBIKYO 54 (2), 62-66, 2019-08-30

    The Japanese Society of Microscopy

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