Observation of dynamic biomolecular processes by high-speed atomic force microscopy

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  • ANDO Toshio
    School of Mathematics and Physics, Kanazawa University

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  • 高速原子間力顕微鏡（AFM）によるたんぱく質分子の観察
  • コウソク ゲンシカンリョク ケンビキョウ AFM ニ ヨル タンパクシツ ブンシ ノ カンサツ

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<p>Biological molecules exhibit various dynamic phenomena in aqueous solutions, such as physiological functions, self-assemblies, and structural transformations. The atomic force microscope (AFM), which has a unique capability of allowing the observation of nanometer-scale objects in liquids, has the potential of capturing these phenomena.　However, the scan speed of AFM was low and hence could not trace fast dynamic molecular processes. This problem had remained unsolved until recently. We developed a high-speed AFM capable of capturing an image at 33 frames/s and succeeded in imaging dynamic phenomena in biological molecules. In this review, the instrumentation is first described briefly and then details of some of the observed phenomena, including the process of planar lipid bilayer formation, the dynamic behavior of point defects in two-dimensional protein crystals and the undulation of unstructured domains of proteins, are explained.</p>

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  • Oyo Buturi

    Oyo Buturi 77 (10), 1181-1191, 2008-10-10

    The Japan Society of Applied Physics

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