Effects of salt concentration on spermidine-induced DNA folding transition

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  • スペルミジンによるDNA凝縮転移に対する塩濃度の影響
  • スペルミジン ニ ヨル DNA ギョウシュク テンイ ニ タイスル エン ノウド ノ エイキョウ

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The change on the higher order structure of individual giant double-strand DNA molecules as a function of salt and spermidine concentrations was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1×10^<-6>-10^<-1>M. From the time successive fluorescent image, we evaluated the long-axis length and the hydrodynamic radius. I propose a scheme for two types of the folding transition manner of single DNAs.

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