The prospect of direct coupling of immunoaffinity capture and capillary isoelectric focusing in analysis of protein drugs

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  • Shimura Kiyohito
    Division of Advanced Chemistry, School of Medicine, Fukushima Medical University

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  • 免疫アフィニティー捕捉とキャピラリー等電点電気泳動を一体化した分析法のタンパク質医薬分析における可能性

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<p>Capillary isoelectric focusing efficiently analyze heterogeneity of protein drugs. My colleagues and I recently developed a method to couple immunoaffinity capture and separation by isoelectric focusing in a single capillary. The method allows sample pretreatment, i.e., purification and concentration, in a single capillary, in which subsequent separation by isoelectric focusing is achieved. The method adds a new option for the analysis of protein drugs in their production processes and for pharmacokinetics in drug-administered patients, with high precision and a minimum amount of samples using an automated instrument.</p>

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