Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
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- Morono Yuki
- Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC
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- Hoshino Tatsuhiko
- Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC
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- Terada Takeshi
- Marine Works Japan Ltd.
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- Suzuki Taketo
- Koken Ltd.
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- Sato Takahiro
- Koken Ltd.
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- Yuasa Hisashi
- Koken Ltd.
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- Kubota Yuji
- Koken Ltd.
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- Inagaki Fumio
- Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC Research and Development Center for Ocean Drilling Science, JAMSTEC
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Abstract
<p>Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.</p>
Journal
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- Microbes and Environments
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Microbes and Environments 33 (2), 222-226, 2018
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
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Details 詳細情報について
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- CRID
- 1390001288043146752
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- NII Article ID
- 130007393763
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- NII Book ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL BIB ID
- 029061169
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- PubMed
- 29910221
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed