Durable Transgene Expression Driven by CpG-Free and -Containing Promoters in Plasmid DNA with CpG-Free Backbone

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  • Suzuki Tetsuya
    Graduate School of Biomedical and Health Sciences, Hiroshima University
  • Goda Takuya
    Graduate School of Biomedical and Health Sciences, Hiroshima University
  • Kamiya Hiroyuki
    Graduate School of Biomedical and Health Sciences, Hiroshima University

書誌事項

公開日
2018-09-01
資源種別
journal article
DOI
  • 10.1248/bpb.b18-00342
公開者
公益社団法人 日本薬学会

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説明

<p>The duration of transgene expression from plasmid DNAs is important for gene therapy with nonviral vectors. In the present study, various cytosine-phosphate-guanine (CpG)-free and CpG-containing transcription regulatory sequences were introduced into plasmid DNAs with a CpG-free backbone. The transgenes encoding mouse secreted alkaline phosphatase and Gaussia princeps luciferase, which are both apparently non-immunogenic, were used as reporters. The plasmid DNAs were injected by the hydrodynamics-based method, and the expression was monitored for 28 d. All transcription regulatory sequences achieved long-term expression, with different expression levels depending on the sequences themselves. These results suggested that durable transgene expression at the proper level can be achieved with plasmid DNAs containing the CpG-free backbone.</p>

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