Micro-computed tomography for evaluating alveolar bone resorption induced by hyperocclusion
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- Tsutsumi Takashi
- Department of Oral Rehabilitation, Fukuoka Dental College
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- Kajiya Hiroshi
- Department of Physiological Science and Molecular Biology, Fukuoka Dental College
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- Tsuzuki Takashi
- Department of Oral Rehabilitation, Fukuoka Dental College
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- Goto Kazuko T.
- Department of Dental Hygiene, Fukuoka College of Health Sciences
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- Okabe Koji
- Department of Physiological Science and Molecular Biology, Fukuoka Dental College
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- Takahashi Yutaka
- Department of Oral Rehabilitation, Fukuoka Dental College
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説明
<p>Purpose: Occlusal trauma, resulting in the destruction of alveolar bone, is a form of periodontal disease caused by excessive mechanical stress (MS) during hyperocclusion. Previously, we showed that C--C chemokine ligand (CCL) 2/CCR2 receptor axis plays a crucial role in MS-dependent osteoclastogenesis. However, in the previous work, we were unable to precisely measure changes in alveolar bone profiles. In the present study, we sought to establish a precise method for evaluating alveolar bone resorption induced by hyperocclusion using micro-computed tomography.</p><p>Methods: Under anesthesia, a stainless steel wire was attached to the molars of 5-week-old C57/BL6 wildtype (WT) mice, CCL2-/- mice, and CCR2-/-mice to induce occlusal force overload. At days 0 and 7, hard tissue samples were harvested and analyzed by micro-computed tomography.</p><p>Results: In the WT mice, bone mineral density of the alveolar bone was significantly decreased at day 7 as compared with day 0, with marked alveolar bone resorption observed. Similarly, significant alveolar bone resorption was observed in the CCL2-/- and CCR2-/- mice at day 7 as compared with day 0.</p><p>Conclusions: Micro-computed tomographic images can be used to measure changes in bone mineral density in a mouse model of hyperocclusion. This method may be useful for further investigating bone changes in other periodontal disease research fields.</p>
収録刊行物
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- Journal of Prosthodontic Research
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Journal of Prosthodontic Research 62 (3), 298-302, 2018
公益社団法人 日本補綴歯科学会
