Isolation of halophilic strains induced by ethyl methanesulfonate in <i>Rhizopogon roseolus</i>
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- GAO Qi
- The United Graduate School of Agricultural Sciences
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- NAKANO Shota
- The United Graduate School of Agricultural Sciences
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- AIMI Tadanori
- Faculty of Agriculture, Tottori University
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- SHIMOMURA Norihiro
- Faculty of Agriculture, Tottori University
Bibliographic Information
- Other Title
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- Ethyl methanesulfonate によって誘導されたショウロの好塩性菌株の分離
- Isolation of halophilic strains induced by ethyl methanesulfonate in Rhizopogon roseolus
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Description
Rhizopogon roseolus is an ectomycorrhizal mushroom with a worldwide distribution. A recent study reported that a salt-tolerant strain could be produced by cross-breeding this mushroom. However, no studies on the selection of salt-tolerant strains by mutagenesis have been performed. Here, to induce mutations, we treated basidiospores of R. roseolus with ethyl methanesulfonate (EMS), a widely used chemical mutagen, and evaluated the salt tolerance of the recovered mutants. Out of 88 monosporous strains recovered from EMS-treated basidiospores, we selected 5 halophilic strains that showed vigorous mycelial growth on medium containing 300 mM NaCl. Three hybrid strains produced by crossing among halophilic monosporous strains also showed vigorous growth on medium containing 300 mM NaCl. To investigate stability of halophilic characteristics, subculturing was carried out for 6 months. After subculturing, halophilicity was recognized in 2 of the 3 hybrid strains. The 2 halophilic hybrid strains were considered suitable for environments containing NaCl.
Journal
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- Mushroom Science and Biotechnology
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Mushroom Science and Biotechnology 24 (4), 182-186, 2016
Japanese Society of Mushroom Science and Biotechnology
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Details 詳細情報について
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- CRID
- 1390001288071089408
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- NII Article ID
- 130007487809
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- NII Book ID
- AA12415767
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- ISSN
- 24327069
- 13487388
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- NDL BIB ID
- 028005402
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed